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Science Investigatory Project EssayAbstractThe ananas is adept of the leading products in the Philippines, as it thrives in tropical climates. However, the pineapple peelings have low commercial value and are therefore merely thrown away, contributing to the Philippines waste problem. This study entitled Saccharification of Pineapple Ananas comosus peelings through Dilute Acid Hydrolysis was conducted to know the ducking of swag content which can be used for other purposes like bioethanol.Three samples were used, each sample was composed of both trials S1T1 (60 spirit level Celsius for 30 min. ), S1T2 (60 degree Celsius for 60 min.), S2T1 (70 degree Celsius for 30 min.), S2T2 (70 degree Celsius for 60 min.), S3T1 (80 degree Celsius for 30 min.), S3T2 (80 degree Celsius for 60 min). The phenol- sulfuric acrid mode was used to suss out the concentration of sugar content present in the substrate. The testify revealed that the dilute acid hydrolysis is an effective way and can saccharify pineapple peelings based on the standard curve. However, the temperature and time was found out that they both have no significant difference in yielding great concentration of sugar as what as the statistical analysis revealed using Kruskal-Wallis test and Mann Whitney U-test.INTRODUCTIONBackground of the studyPineapple (Ananas comosus) is the common name for a tropical plant and its edible fruit which are coalesced berries. Pineapples are the further bromeliad fruit in widespread cultivation. It can be grown as an ornamental, especially from the leafy tops. Some sources say that the plant go forth flower later about 24 months produce a fruit during the following six months while others indicate a 20-month timetable. Pineapple is eaten fresh or canned or juiced. It is popularly used in desserts, salads, as a complement to meat dishes and infruit cocktail. The popularity of the pineapple is due to its sweet-sour taste containing 15% sugar and malic and citric fr uit acids. It is in like manner high in vitamin B1, B2, B6 and C. Its protein-digesting enzyme bromelain seems to help digestion at the end of a high protein meal. In the Philippines, pineapple leaves are used as the source of a cloth fiber called pia. The pineapple is a herbaceous short-lived perennial plant which grows to 1.0 to 1.5 metres (3.3 to 4.9 ft) tall. The plant only produces one fruit and then dies. Commercially suckers that get along around the base are cultivated.It has 30 or more long, narrow, fleshy, trough-shaped leaves with sharp spines along the margins that are 30 to 100 centimetres (1.0 to 3.3 ft) long, surrounding a thick stem. In the first year of growth the axis lengthens and thickens, bearing numerous leaves in close spirals. After 12 to 20 months the stem grows into a spike-like inflorescence up to 15 cm long with over 100 spirally arranged, trimerous flowers, each subtended by a bract. Flower colours vary, depending on variety, from lavender, through li ght discolour to red. The ovaries develop into berries which coalesce into a large, compact, multiple accessory fruit. The fruit of a pineapple is arranged in two interlocking helices, eight in one direction, thirteen in the other, each being a Fibonacci number. (http//en.wikipedia.org/wiki/Pineapple) Saccharification is the process of breaking a complex carbohydrate into its monosaccharide components.It is the hydrolysis of carbohydrates such as cellulose and starch. It increases hydrolysis rates by reducing product inhibition of enzymes and reduces tank usage by combining the processes into one (http//www.merriam-webster.com/dictionary/saccharification) Phenol Sulfuric Acid Method is an example of a colorimetric method that is widely used to determine the total concentration of carbohydrates present in foods. A clear aqueous firmness of purpose of carbohydrates to be canvas is placed in a test tube, then phenol and sulfuric acid are added.The solution turns a yellow- orange col or as a result of the interaction between the carbohydrates and phenol.The sulfuric acid causes all non- reducing sugars to be converted to reducing sugars so that this method determines the total sugar present. This method is non- stoichemetric and so it is necessary to prepare a calibration curve using a series of standard known carbohydrate concentration (http//www.merriam-webster.com/dictionary/phenol-sulfuric acid method). Dilute Acid Hydrolysis is a process of hydrolyzing lignocellulosic materials by subjecting dried lignocellulosic material in a reactor to a catalyst comprised of a dilute solution of a strong acid to lower the activation energy of cellulose hydrolysis and ultimately obtain higher sugar yields (http//www.merriam-webster.com/dictionary/dilute acid hydrolysis).Statement of the puzzleThis study aims to saccharify pineapple Ananas comosus peelings through dilute acid hydrolysis. Specifically, the study attempts1. To know whether the dilute acid hydrolysis is an e ffective way of yielding concentration of sugar from pineapple peelings 2. To determine whether the temperature has an effect on the concentration of sugar 3. To determine whether the length of time of reacion can affect the concentration of sugar that will be obtained. Hypothesis1. The dilute acid hydrolysis is an effective way of yielding a greater concentration of sugar. 2. The higher the temperature of the solution, the greater the concentration. 3. The longer the time of reaction, the greater the concentration of sugar that will be obtained.Significance of the studyOn average, 435,000 metric tons of pineapples are produced annually in the Philippines, which is one of the countrys leading commercial fruit products. However, there are a lot of unused excess parts of the pineapple, notably the peelings, which are considered as waste and fetch to the countrys garbage problem.This study aims to utilize pineapple peelings as a substrate for Saccharification process through dilute ac id hydrolysis. By elbow room of this, the concentration of sugar present in this biomass can be determined which can be use for other purposes and for other studies such as producing bioethanol or even biofuel.Scope and LimitationThis study is limited to the use of pineapple Ananas comosus peelings as a substrate for the saccharification process. The peel samples were bought from the market. The experiment was conducted at chemical Engineering where the chemicals and other laboratory materials were obtained located at University of the Philippines at Los Baos, Laguna from November to December 2010. The study focuses only on the determination of the concentration of sugar present in the pineapple peelings.Definition of TermsAbsorbance- is defined as the ratio of the radiant flux absorbed by a body to that attendant upon it. Glucose- is called as a simple sugar or monosaccharideSaccharification the process of breaking a complex carbohydrate (as starch or cellulose) into its monosa ccharide components Spectrophotometer- consists of two instruments, namely a spectrometer for producing light of any selected color (wavelength), and a photometer for measuring the intensity of light. Sulfuric acid (H2SO4) is a clear, colorless, odorless, viscous transparent that is very corrosive. Sugar- is a term for a class of edible crystalline carbohydrates, mainly sucrose, lactose, and fructose characterized by a sweet flavor. In food, sugar close exclusively refers to sucrose, which primarily comes from sugar cane and sugar beet. Standard curve- is a quantitative research tool, a method of plotting assay data that is used to determine the concentration of a substance. It can be used in many biological experiments.